Use of Metagenomic Whole Genome Shotgun Sequencing Data in Taxonomic Assignment of Dipterygium glaucum Rhizosphere and Surrounding Bulk Soil Microbiomes, and Their Response to Watering

The metagenomic whole genome shotgun sequencing (mWGS) approach was used to detect signatures of the rhizosphere microbiomes Dipterygium glaucum and surrounding bulk soil microbiomes, differential microbial responses due watering. Preliminary results reflect reliability experiment rationality grouping microbiomes. Based on abundance non-redundant genes, bacterial genomes showed highest level, followed by Archaeal Eukaryotic genomes, then, least abundant viruses. Overall indicate that most members bacteria have a higher abundance/relative (AB/RA) pattern in towards plant growth promotion, while eukaryota soil, likely acting as pathogens. also contribution mycorrhiza (genus Rhizophagus) mediating complex mutualistic associations between microbes (either beneficial or harmful) roots. Some these symbiotic relationships involve different domains responding differentially root exudates. Among are included genus Burkholderia eukaryotic Trichoderma, which antagonistic activities against Fusarium. Another example involves Ochrobactrum phage POA1180, its host One major challenges nutrition other manipulate nitrogen levels soil. perform contraversal actions fixation (the methanogen Euryarchaeota) ammonia oxidation (Crenarchaeota). net level is originally based AB/RA partially environmental condition. Watering seems influence large number microbes, where drought-sensitive (members phyla Acidobacteria Gemmatimonadetes) an increased after watering, others (Burkholderia Trichoderma) seem be among assisting plants withstand abiotic stresses. This study sheds light efficient use mWGS taxonomic assignment their response It provides new avenues for improving biotic resistance domestic germplasm via manipulation microbes.